Gas Chromatography and Lipids - Chapter 5
Table 5.1Equivalent chain lengths and relative retention times of some unsaturated esters on packed columns with EGSS-X, EGSS-Y and SILAR 10C as stationary phases. |
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| Methyl ester | ECLs | Relative Retention Times* | ||||
|---|---|---|---|---|---|---|
| EGSS-X | EGSS-Y | Silar 10C | EGSS-X | EGSS-Y | Silar 10C | |
| 16:0 | 16.00 | 16.00 | 16.00 | 0.58 | 0..57 | 0.67 |
| 16:1(n-9) | 16.57 | 16.62 | 17.08 | 0.69 | 0.68 | 0.84 |
| 16:2(n-6) | 17.65 | 17.45 | - | 0.90 | 0.85 | - |
| 18:0 | 18.00 | 18.00 | 18.00 | 1.00 | 1.00 | 1.00 |
| 18:1(n-9) | 18.53 | 18.52 | 19.00 | 1.19 | 1.16 | 1.23 |
| 18:2(n-6) | 19.42 | 19.20 | 20.07 | 1.52 | 1.41 | 1.56 |
| 18:3(n-6) | 20.00 | 19.67 | 21.10 | 1.80 | 1.60 | 1.75 |
| 18:3(n-3) | 20.40 | 20.02 | 21.28 | 2.02 | 1.78 | 2.01 |
| 18:4(n-3) | 21.05 | 20.52 | 22.12 | 2.40 | 2.04 | 2.37 |
| 20:0 | 20.00 | 20.00 | 20.00 | 1.77 | 1.76 | 1.53 |
| 20:1(n-9) | 20.50 | 20.45 | 20.90 | 2.08 | 2.01 | 1.84 |
| 20:2(n-6) | 21.40 | 21.15 | - | 2.67 | 2.44 | - |
| 20:3(n-9) | 21.63 | 21.33 | 22.22 | 2.87 | 2.57 | 2.22 |
| 20:3(n-6) | 21.77 | 21.53 | 22.72 | 2.99 | 2.72 | 2.42 |
| 20:3(n-3) | 21.95 | 21.60 | 23.10 | 3.13 | 2.78 | 2.64 |
| 20:4(n-6) | 22.43 | 22.00 | 23.53 | 3.59 | 3.10 | 3.14 |
| 20:4(n-3) | 23.00 | 22.47 | 24.13 | 4.18 | 3.54 | 3.55 |
| 20:5(n-3) | 23.50 | 22.80 | 24.77 | 4.85 | 3.91 | 4.06 |
| 22:0 | 22.00 | 22.00 | 22.00 | 3.14 | 3.12 | 2.31 |
| 22:1(n-9) | 22.43 | 22.35 | 22.80 | 3.59 | 3.44 | 2.71 |
| 22:4(n-6) | 24.45 | 24.00 | 25.70 | 6.34 | 5.48 | 4.94 |
| 22:5(n-6) | 24.57 | 23.85 | 25.96 | 6.55 | 5.27 | 5.14 |
| 22:5(n-3) | 25.53 | 24.80 | 26.83 | 8.60 | 6.92 | 6.20 |
| 22:6(n-3) | 26.18 | 25.50 | 27.40 | 9.10 | 7.74 | 6.89 |
| 24:0 | 24.00 | 24.00 | 24.00 | 5.59 | 5.50 | 3.50 |
| * relative to 18:0 | ||||||
| EGSS-X and EGSS-Y: Data obtained with 2m x 4mm glass columns packed with 15% (w/w) stationary phase on Chromosorb W (100-120 mesh), acid-washed and silanized). Carrier gas, nitrogen at 50 mL/min.; column temperature, 178ºC (EGSS-X) and 194ºC (EGSS-Y). Silar 10C: Data obtained with 3m x 4mm glass columns packed with 10% (w/w) stationary phase on Gaschrom Q (100-120 mesh). Carrier gas, nitrogen at 11.5 mL/min; column temperature, 210ºC. |
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This document is part of the book "Gas Chromatography and Lipids" by William W. Christie and published by the Oily Press, Bridgwater in 1989.
Updated: 12/7/2006 |
Scottish Crop Research Institute (and MRS Lipid Analysis Unit), Invergowrie, Dundee (DD2 5DA), Scotland |
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