The Lipid Library

GAS CHROMATOGRAPHY AND LIPIDS

The following web pages in HTML format are the text of the book "Gas Chromatography and Lipids" by William W. Christie and published in 1989 by the Oily Press Ltd. The book is now out of print, and is republished here in an html version by kind permission of P.J. Barnes & Associates. Apart from trivial corrections here and there, or links to pages elsewhere on this site, the contents of the book remain as they were in the original. While I have been pleasantly surprised how little this has dated, my latest book "Lipid Analysis - 3rd Edition" (2003) is certainly more up-to-date as well as being much more comprehensive in terms of applications to lipids in general. If you have problems loading the larger files (Internet Explorer Version 6 and earlier), click on the 'Refresh' button. A PDF file of the book is available as a free download from the Oily Press Ltd.

Bill Christie

• Chapter 1.  Introduction and Summary
• Chapter 2.  Fatty acids and Lipids: Structures, Extraction and Fractionation into Classes
                       Sections A to D.  Lipid structures
                       Sections E and F.  Extraction and fractionation into lipid classes
• Chapter 3.  Gas chromatography: Theoretical Aspects and Instrumentation    

• Chapter 4.  The Preparation of Derivatives of Fatty Acids
• Chapter 5.  Gas Chromatographic Analysis of Fatty Acid Derivatives
                       Sections A to D.  Instrumentation and identification via retention data
                       Sections E to G.  Applications to natural samples and quantification
• Chapter 6.  Isolation of Fatty Acids and Identification by Spectroscopic and Chemical Degradative Techniques
                       Sections A and B.  Isolation of fatty acids
                       Sections C and D.  Identification by spectroscopy and chemical degradation
• Chapter 7.  Gas Chromatography-Mass Spectrometry and Fatty Acids

• Chapter 8.  Gas Chromatographic Analysis of Molecular Species of Lipids
                       Sections A and B.  Introduction and high-temperature gas chromatography of triacylglycerols
                       Sections C to G.  GC of complex glycerolipids and other simple lipids
                       Sections H and I.  GC of sphingolipids and of total lipid extracts
• Chapter 9.  Alternative or Complementary Methods for the Analysis of Molecular Species of Lipids
• Chapter 10.  Some Miscellaneous Separations of Lipids by Gas Chromatography

• References

Please note! Many of the procedures used in the analysis of lipids require the use of solvents and other hazardous materials. We cannot accept any responsibility for accidents that may occur while using methods described in this book.

Updated: 12/8/2007 Credits/disclaimer	Scottish Crop Research Institute (and MRS Lipid Analysis Unit), Invergowrie, Dundee (DD2 5DA), Scotland